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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [8]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF449 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse LIF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human (rh) LIF is observed. In direct ELISAs, no cross-reactivity with rhCNTF, recombinant mouse (rm) G-CSF, rmIL-6, and rmOSM is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Direct Conversion of Mouse Fibroblasts into Neural Stem Cells by Chemical Cocktail Requires Stepwise Activation of Growth Factors and Nup210.
TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCs.
Leukemia inhibitory factor signaling is required for lung protection during pneumonia.
Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity.
Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune disease.
Astrocytes promote myelination in response to electrical impulses.
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
Tang Y, Xiong S, Yu P, Liu F, Cheng L
Cell reports 2018 Jul 31;24(5):1355-1362.e3
Cell reports 2018 Jul 31;24(5):1355-1362.e3
TLR9 expression and secretion of LIF by prostate cancer cells stimulates accumulation and activity of polymorphonuclear MDSCs.
Won H, Moreira D, Gao C, Duttagupta P, Zhao X, Manuel E, Diamond D, Yuan YC, Liu Z, Jones J, D'Apuzzo M, Pal S, Kortylewski M
Journal of leukocyte biology 2017 Aug;102(2):423-436
Journal of leukocyte biology 2017 Aug;102(2):423-436
Leukemia inhibitory factor signaling is required for lung protection during pneumonia.
Quinton LJ, Mizgerd JP, Hilliard KL, Jones MR, Kwon CY, Allen E
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 15;188(12):6300-8
Journal of immunology (Baltimore, Md. : 1950) 2012 Jun 15;188(12):6300-8
Adenosine A2B receptor-mediated leukemia inhibitory factor release from astrocytes protects cortical neurons against excitotoxicity.
Moidunny S, Vinet J, Wesseling E, Bijzet J, Shieh CH, van Ijzendoorn SC, Bezzi P, Boddeke HW, Biber K
Journal of neuroinflammation 2012 Aug 16;9:198
Journal of neuroinflammation 2012 Aug 16;9:198
Leukemia inhibitory factor inhibits T helper 17 cell differentiation and confers treatment effects of neural progenitor cell therapy in autoimmune disease.
Cao W, Yang Y, Wang Z, Liu A, Fang L, Wu F, Hong J, Shi Y, Leung S, Dong C, Zhang JZ
Immunity 2011 Aug 26;35(2):273-84
Immunity 2011 Aug 26;35(2):273-84
Astrocytes promote myelination in response to electrical impulses.
Ishibashi T, Dakin KA, Stevens B, Lee PR, Kozlov SV, Stewart CL, Fields RD
Neuron 2006 Mar 16;49(6):823-32
Neuron 2006 Mar 16;49(6):823-32
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
Archives of dermatological research 2001 Nov;293(10):508-14
Archives of dermatological research 2001 Nov;293(10):508-14
The IL-6 family cytokines, interleukin-6, interleukin-11, oncostatin M, and leukemia inhibitory factor, enhance mast cell growth through fibroblast-dependent pathway in mice.
Gyotoku E, Morita E, Kameyoshi Y, Hiragun T, Yamamoto S, Hide M
Archives of dermatological research 2001 Nov;293(10):508-14
Archives of dermatological research 2001 Nov;293(10):508-14
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-6 Secretion Induced by LIF and Neutralization by Mouse LIF Antibody. Recombinant Mouse LIF (Catalog # 8878-LF) stimulates IL-6 secretion in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line) as measured by the Mouse IL-6 Quantikine ELISA (Catalog # M6000B). IL-6 secretion elicited by 3 ng/mL Recombinant Mouse LIF is neutralized (green line) by increasing concentrations of Goat Anti-Mouse LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF449). The ND50 is typically 10-60 ng/mL.
