MA5-23809

antibody from Invitrogen Antibodies

Targeting: LIF CDF, DIA, HILDA

Immunohistochemistry (Recommended by provider)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-23809

Provider

Invitrogen Antibodies

Product name

LIF Monoclonal Antibody (9824)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

In sandwich ELISAs, no significant cross-reactivity or interference with recombinant human (rh) IL-1 alpha, rhIL-1 beta, rhIL-2, rhIL-3, rhIL-4, rhIL-6, rhIL-7, rhIL-8, rhG-CSF, rhGM-CSF, rhOSM, rhTGF-beta 1, rhTNF-alpha, rhTNF-beta, recombinant mouse (rm) IL-1 beta, rmIL-3, rmIL-4, rmIL-5, rmIL-6, rmIL-7, rmGM-CSF, bovine (b) FGF acidic, bFGF basic, human (h) PDGF, porcine (p) PDGF, hTGF-beta 1, pTGF-beta 1.2, or pTGF-beta 2 is observed. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

9824

Vial size

100 µg

Concentration

0.5 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of LIF in TF‚1 human erythroleukemic cell line. Samples were incubated in LIF monoclonal antibody (Product # MA5-23809). Recombinant Human LIF stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by 1.5 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Mouse Anti-Human LIF Monoclonal Antibody. The ND50 is typically 0.06-0.2 µg/mL.