PA5-47337

antibody from Invitrogen Antibodies

Targeting: LIF CDF, DIA, HILDA

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-47337

Provider

Invitrogen Antibodies

Product name

LIF Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

In direct ELISAs and Western blots, less than 30% cross-reactivity with recombinant mouse LIF is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LIF in immersion fixed paraffin-embedded sections of human lung array. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337) using a dilution of 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LIF in immersion fixed paraffin-embedded sections of human Alzheimers brain. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337) using a dilution of 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic . Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of LIF in TF‚1 human erythroleukemic cell line. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337). Recombinant Human LIF stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin. Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.04‚0.08 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Neutralization of LIF in TF‚1 human erythroleukemic cell line. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337). Recombinant Human LIF stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin. Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.04‚0.08 µg/mL.