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Immunohistochemistry
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- PA5-47337 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- LIF Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 30% cross-reactivity with recombinant mouse LIF is observed. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Neutralization of LIF in TF‚1 human erythroleukemic cell line. Samples were incubated in LIF polyclonal antibody (Product # PA5-47337). Recombinant Human LIF stimulates proliferation in the TF‚1 human erythroleukemic cell line in a dose-dependent manner (orange line), as measured by Resazurin. Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.04‚0.08 µg/mL.
