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- Product number
- NBP1-76554 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76554, RRID:AB_11014546
- Product name
- Rabbit Polyclonal LIF Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. At least two isoforms of LIF are known to exist.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Lewis lung carcinoma regulation of mechanical stretch-induced protein synthesis in cultured myotubes.
Gao S, Carson JA
American journal of physiology. Cell physiology 2016 Jan 1;310(1):C66-79
American journal of physiology. Cell physiology 2016 Jan 1;310(1):C66-79
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIF Antibody [NBP1-76554] - 3T3 cell lysate with LIF antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Rat Lung Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Mouse Cell Lines and Tissue. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation with Recombinant Protein. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1: 1 ug/mL, 2: 2ug/mL, 3: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: LIF Antibody [NBP1-76554] - Western Blot Validation in Human Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: LIF NBP1-76554, (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
