Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-250-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human LIF Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human LIF in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse LIF is observed. Neutralizes the biological activity of recombinant human LIF and will also neutralize the biological activity of recombinant mouse LIF at a 50-fold higher IgG concentration.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P15018
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Leukemia inhibitory factor functions in parallel with interleukin-6 to promote ovarian cancer growth.
McLean K, Tan L, Bolland DE, Coffman LG, Peterson LF, Talpaz M, Neamati N, Buckanovich RJ
Oncogene 2019 Feb;38(9):1576-1584
Oncogene 2019 Feb;38(9):1576-1584
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF (Catalog # 7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line) as measured by Resazurin (Catalog # AR002). Proliferation elicited by 0.5 ng/mL Recom-binant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Polyclonal Antibody (Catalog # AB-250-NA). The ND50 is typically 0.1-0.4 µg/mL.