Antibody data
- Antibody Data
- Antigen structure
- References [15]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Blocking/Neutralizing [1]
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- Product number
- AF-250-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human LIF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human LIF in direct ELISAs. In direct ELISAs, less than 45% cross-reactivity with recombinant mouse LIF and less than 15% cross-reactivity with recombinant rat LIF is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P15018
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation.
Senescent fibroblasts drive ageing pigmentation: A potential therapeutic target for senile lentigo.
Possible Involvement of Human Mast Cells in the Establishment of Pregnancy via Killer Cell Ig-Like Receptor 2DL4.
Decreased MUC1 in endometrium is an independent receptivity marker in recurrent implantation failure during implantation window.
Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of patients with adenomyosis during implantation window.
Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
Effect of levonorgestrel and mifepristone on endometrial receptivity markers in a three-dimensional human endometrial cell culture model.
Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression.
Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
Leukemia inhibitory factor regulates prolactin secretion in prolactinoma and lactotroph cells.
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Kessler M, Hoffmann K, Fritsche K, Brinkmann V, Mollenkopf HJ, Thieck O, Teixeira da Costa AR, Braicu EI, Sehouli J, Mangler M, Berger H, Meyer TF
Nature communications 2019 Mar 18;10(1):1194
Nature communications 2019 Mar 18;10(1):1194
Senescent fibroblasts drive ageing pigmentation: A potential therapeutic target for senile lentigo.
Yoon JE, Kim Y, Kwon S, Kim M, Kim YH, Kim JH, Park TJ, Kang HY
Theranostics 2018;8(17):4620-4632
Theranostics 2018;8(17):4620-4632
Possible Involvement of Human Mast Cells in the Establishment of Pregnancy via Killer Cell Ig-Like Receptor 2DL4.
Ueshima C, Kataoka TR, Hirata M, Sugimoto A, Iemura Y, Minamiguchi S, Nomura T, Haga H
The American journal of pathology 2018 Jun;188(6):1497-1508
The American journal of pathology 2018 Jun;188(6):1497-1508
Decreased MUC1 in endometrium is an independent receptivity marker in recurrent implantation failure during implantation window.
Wu F, Chen X, Liu Y, Liang B, Xu H, Li TC, Wang CC
Reproductive biology and endocrinology : RB&E 2018 Jun 21;16(1):60
Reproductive biology and endocrinology : RB&E 2018 Jun 21;16(1):60
Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of patients with adenomyosis during implantation window.
Xiao Y, Sun X, Yang X, Zhang J, Xue Q, Cai B, Zhou Y
Fertility and sterility 2010 Jun;94(1):85-9
Fertility and sterility 2010 Jun;94(1):85-9
Disturbances in the LIF pathway in the endometrium among women with unexplained infertility.
Aghajanova L, Altmäe S, Bjuresten K, Hovatta O, Landgren BM, Stavreus-Evers A
Fertility and sterility 2009 Jun;91(6):2602-10
Fertility and sterility 2009 Jun;91(6):2602-10
Effect of levonorgestrel and mifepristone on endometrial receptivity markers in a three-dimensional human endometrial cell culture model.
Meng CX, Andersson KL, Bentin-Ley U, Gemzell-Danielsson K, Lalitkumar PG
Fertility and sterility 2009 Jan;91(1):256-64
Fertility and sterility 2009 Jan;91(1):256-64
Leukemia inhibitory factor functions as a growth factor in pancreas carcinoma cells: Involvement of regulation of LIF and its receptor expression.
Kamohara H, Ogawa M, Ishiko T, Sakamoto K, Baba H
International journal of oncology 2007 Apr;30(4):977-83
International journal of oncology 2007 Apr;30(4):977-83
Expression of leukemia inhibitory factor and its receptors is increased during differentiation of human embryonic stem cells.
Aghajanova L, Skottman H, Strömberg AM, Inzunza J, Lahesmaa R, Hovatta O
Fertility and sterility 2006 Oct;86(4 Suppl):1193-209
Fertility and sterility 2006 Oct;86(4 Suppl):1193-209
The N-terminal cytokine binding domain of LIFR is required for CNTF binding and signaling.
He W, Gong K, Smith DK, Ip NY
FEBS letters 2005 Aug 15;579(20):4317-23
FEBS letters 2005 Aug 15;579(20):4317-23
Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
Andersson By U, Tani E, Andersson U, Henter JI
Journal of pediatric hematology/oncology 2004 Nov;26(11):706-11
Journal of pediatric hematology/oncology 2004 Nov;26(11):706-11
Tumor necrosis factor, interleukin 11, and leukemia inhibitory factor produced by Langerhans cells in Langerhans cell histiocytosis.
Andersson By U, Tani E, Andersson U, Henter JI
Journal of pediatric hematology/oncology 2004 Nov;26(11):706-11
Journal of pediatric hematology/oncology 2004 Nov;26(11):706-11
Leukemia inhibitory factor regulates prolactin secretion in prolactinoma and lactotroph cells.
Ben-Shlomo A, Miklovsky I, Ren SG, Yong WH, Heaney AP, Culler MD, Melmed S
The Journal of clinical endocrinology and metabolism 2003 Feb;88(2):858-63
The Journal of clinical endocrinology and metabolism 2003 Feb;88(2):858-63
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Sengupta J, Dhawan L, Ghosh D
Cytokine 2003 Dec 21;24(6):277-85
Cytokine 2003 Dec 21;24(6):277-85
Immunohistochemical localization of leukemia inhibitory factor, interleukins 1 and 6 at the primary implantation site in the rhesus monkey.
Sengupta J, Dhawan L, Ghosh D
Cytokine 2003 Dec 21;24(6):277-85
Cytokine 2003 Dec 21;24(6):277-85
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LIF in Human Alzheimer's Brain. LIF was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- LIF in Human Lung. LIF was detected in immersion fixed paraffin-embedded sections of human lung array using Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by LIF and Neutralization by Human LIF Antibody. Recombinant Human LIF (Catalog # 7734-LF) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-depend-ent manner (orange line), as measured by Resazurin (Catalog # AR002). Proliferation elicited by 3 ng/mL Recombinant Human LIF is neutralized (green line) by increasing concentrations of Goat Anti-Human LIF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-250-NA). The ND50 is typically 0.04-0.08 µg/mL.