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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-97133 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- GDI2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: QVIRVICILS HPIKNTNDAN SCQIIIPQNQ VNRKSDIYVC MISFAHNVAA QGKYIAIVST TVETKEPEKE IRPALELLEP IEQKFVSISD LLVPKDLGTE SQIFISRTYD ATTHFETTCD DIKNIYKRMT GSEFDFEEMK RKKNDIYGED; Positive Samples: HT-29, A-549, HepG2, U-87MG, Mouse spleen, Mouse kidney, Mouse lung, Rat liver, Rat spinal cord; Cellular Location: Cytoplasm, Membrane, Peripheral membrane protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.26 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GDI2 in extracts from normal (control) and GDI2 knockout (KO) 293T cells using GDI2 Polyclonal Antibody (Product # PA5-97133) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of GDI2 in extracts of various cell lines using GDI2 Polyclonal Antibody (Product # PA5-97133) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of GDI2 was performed in NIH/3T3 cells using GDI2 Polyclonal Antibody (Product # PA5-97133) at a dilution of 1:100. Blue: DAPI for nuclear staining.
