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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- NBP2-22365-100uL - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SMPD1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq].
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMPD1 Antibody [NBP2-22365] - Analysis of K562 cell line lysates (35ug/lane). This demonstrates detected the SMPD1 protein (arrow).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMPD1 Antibody [NBP2-22365] - Analysis of human testis tissue followed by peroxidase conjufation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SMPD1 Antibody [NBP2-22365] - All lanes : 042054 (1:4000)| Lane 1: K562 whole cell lysate |Lane 2: MCF-7 whole cell lysate |Lane 3: HepG2 whole cell lysate |Lane 4: A431 whole cell lysate |Lysates/proteins at 20 g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
