PA5-95730

antibody from Invitrogen Antibodies

Targeting: SMPD1 ASM

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-95730

Provider

Invitrogen Antibodies

Product name

ASM Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: THP-1, HepG2, U-251MG, MCF-7, Mouse brain, Mouse liver, Rat brain, Rat liver; Cellular Location: Lysosome Immunogen sequence: PARLHRIVPR LRDVFGWGNL TCPICKGLFT AINLGLKKEP NVARVGSVAI KLCNLLKIAP PAVCQSIVHL FEDDMVEVWR RSVLSPSEAC GLLLGSTCGH WDIFSSWNIS LPTVPKPPPK PPSPPAPGAP VSRILFLTDL HWDHDYLEGT DPDCADPLCC RRGSGLPPAS RPGAGYWGEY SKCDLPLRTL ESLLSGLGPA GPFDMVYWTG DIPAHDVWHQ TRQDQLRALT TVTALVRKFL GPVPVYPAVG NHESTPVNSF PPPFIEGNHS S

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.10 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

References

Submitted references

High levels of modified ceramides are a defining feature of murine and human cancer cachexia.

Morigny P, Zuber J, Haid M, Kaltenecker D, Riols F, Lima JDC, Simoes E, Otoch JP, Schmidt SF, Herzig S, Adamski J, Seelaender M, Berriel Diaz M, Rohm M
Journal of cachexia, sarcopenia and muscle 2020 Dec;11(6):1459-1475

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of ASM was performed in NIH-3T3 cells using ASM Polyclonal Antibody (Product # PA5-95730) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of ASM in NIH-3T3 cells. Samples were incubated with ASM Polyclonal antibody (Product # PA5-95730) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3 Expression of key enzymes involved in sphingolipid metabolism is altered in metabolic organs. ( A , B ) Liver mRNA levels of enzymes involved in ceramide metabolism in phosphate-buffered saline (PBS) (grey bars, n = 8 animals), non-cachectic NC26 (blue bars, n = 7 animals), and cachectic C26 (C26-cx, red bars, n = 6 animals) tumour-bearing mice ( A , experiment 2 ); and PBS (grey bars, n = 10 animals), pre-cachectic (C26-precx, pink bars, n = 11 animals), and cachectic (C26-cx, red bars, n = 9 animals) C26 tumour-bearing mice ( B , experiment 3 ). ( C , D ) Liver protein levels of Cers6 and Smpd1 from experiments 2 and 3 . Vinculin was used as loading control. ( E - I ) mRNA levels of enzymes involved in ceramide metabolism in epididymal white adipose tissue (eWAT) ( E , F ), GC muscle ( G , H ), and tumour ( I ). Data are mean +- SEM; statistical analyses were performed using unpaired one-way ANOVA or Kruskal-Wallis tests with Bonferroni or Dunn's post-hoc tests, respectively. Tests were two sided. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.