Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- F54362 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- SMPD1 Antibody / Acid Sphingomyelinase
- Antibody type
- Polyclonal
- Description
- Antigen affinity purified
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 0.08 µl, 0.4 µl
- Storage
- Aliquot the Acid Sphingomyelinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) K562, 2) MCF7, 3) HepG2 and 4) A431 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) cerebellum, 2) K562, 3) HepG2 and 4) MCF7 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human breast tissue with Acid Sphingomyelinase antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of fixed and permeabilized human K562 cells with Acid Sphingomyelinase antibody; Blue=isotype control, Green= Acid Sphingomyelinase antibody.