F54362

antibody from NSJ Bioreagents

Targeting: SMPD1 ASM

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54362

Provider

NSJ Bioreagents

Product name

Acid Sphingomyelinase Antibody / SMPD1

Antibody type

Polyclonal

Description

This highly specific Acid Sphingomyelinase antibody is suitable for use in Immunohistochemistry/Western blot/Flow cytometry applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the Acid Sphingomyelinase antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) K562, 2) MCF7, 3) HepG2 and 4) A431 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) cerebellum, 2) K562, 3) HepG2 and 4) MCF7 cell lysate with Acid Sphingomyelinase antibody. Predicted molecular weight ~70 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human breast tissue with Acid Sphingomyelinase antibody. HIER: steam section in pH9 EDTA for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of fixed and permeabilized human K562 cells with Acid Sphingomyelinase antibody; Blue=isotype control, Green= Acid Sphingomyelinase antibody.