Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- ABIN955389 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-tyrosyl-DNA phosphodiesterase 2 (TDP2) (C-Term), (AA 253-281) antibody
- Antibody type
- Polyclonal
- Antigen
- KLH conjugated synthetic peptide between 253-281 amino acids from the C-terminal region of Human TTRAP.
- Description
- Affinity Chromatography on Protein A
- Reactivity
- Human
- Host
- Rabbit
- Epitope
- C-Term,AA 253-281
- Vial size
- 0.4 mL
- Concentration
- 0.25 mg/mL
- Storage
- Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references An approach based on a genome-wide association study reveals candidate loci for narcolepsy.
PhiC31 integrase interacts with TTRAP and inhibits NFkappaB activation.
Shimada M, Miyagawa T, Kawashima M, Tanaka S, Honda Y, Honda M, Tokunaga K
Human genetics 2010 Oct;128(4):433-41
Human genetics 2010 Oct;128(4):433-41
PhiC31 integrase interacts with TTRAP and inhibits NFkappaB activation.
Wang BY, Xu GL, Zhou CH, Tian L, Xue JL, Chen JZ, Jia W
Molecular biology reports 2010 Jul;37(6):2809-16
Molecular biology reports 2010 Jul;37(6):2809-16
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Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Western blot analysis of TTRAP Antibody (C-term) Cat.-No AP51353PU-N in A549 cell line lysates (35ug/lane). This demonstrates the EAPII antibody detected the EAPII protein (arrow).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of TTRAP Antibody (C-term) Cat.-No AP51353PU-N with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Formalin fixed and paraffin embedded human prostate carcinoma reacted with TTRAP Antibody (C-term) Cat.-No AP51353PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Supportive validation
- Submitted by
- antibodies-online (provider)
- Main image
- Experimental details
- Flow cytometric analysis of A549 cells using TTRAP Antibody (C-term) Cat.-No AP51353PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.