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Western blot
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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- R31961 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- OSM Antibody / Oncostatin M
- Antibody type
- Polyclonal
- Description
- This highly specific OSM antibody is suitable for use in Western blot/Immunohistochemistry/ELISA applications with human samples.
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the OSM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A549, 2) HUT, 3) Jurkat, 4) SW620 and 5) MCF7 cell lysate with OSM antibody. Expected molecular weight ~28 kDa (precursor), ~24 kDa (pro form), ~28 kDa (glycosylated pro form); observed here at ~40 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestinal cancer tissue with OSM antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
