Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- R31961 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Oncostatin M Antibody / OSM
- Antibody type
- Polyclonal
- Antigen
- Amino acids 26-220 of human Oncostatin M were used as the immunogen for the OSM antibody.
- Description
- Antigen affinity
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- Lyophilized; resuspend with 200 ul for 0.5 mg/ml
- Storage
- After reconstitution, the OSM antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) A549, 2) HUT, 3) Jurkat, 4) SW620 and 5) MCF7 cell lysate with OSM antibody. Expected molecular weight ~28 kDa (precursor), ~24 kDa (pro form), ~28 kDa (glycosylated pro form); observed here at ~40 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human intestinal cancer tissue with OSM antibody. HIER: Boil the paraffin sections in pH 6, 10mM citrate buffer for 20 minutes and allow to cool prior to staining.
