Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MAB2951 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Oncostatin M/OSM Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human Oncostatin M/OSM in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Oncostatin M, recombinant human (rh) CLC, rhCNTF, rhCardiotrophin-1, rhIL-6, rhIL-11, or rhLIF is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P13725
- Isotype
- IgG
- Antibody clone number
- 17022
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Oncostatin M/OSM in Human Dendritic Cells by Flow Cytometry. Human monocyte-derived dendritic cells were stained with Mouse Anti-Human Oncostatin M Monoclonal Antibody (Catalog # MAB2951, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by Oncostatin M/OSM and Neutralization by Human Oncostatin M/OSM Antibody. Recombinant Human Oncostatin M (Catalog # 295-OM) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Prolif-eration elicited by Recom-binant Human Oncostatin M (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human Oncostatin M Monoclonal Antibody (Catalog # MAB2951). The ND50 is typically 0.3-1.5 µg/mL.