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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Flow cytometry [2]
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Validation data
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- Product number
- TA800053 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA800053, RRID:AB_2625180
- Product name
- TRIM9 mouse monoclonal antibody, clone OTI2A1 (formerly 2A1)
- Antibody type
- Monoclonal
- Description
- TRIM9 mouse monoclonal antibody, clone OTI2A1 (formerly 2A1)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TRIM9
- Isotype
- IgG
- Antibody clone number
- OTI2A1
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TRIM9 (RC201423, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TRIM9.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC201423 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-TRIM9 antibody(TA800053), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-TRIM9 antibody(TA800053),(Red), compared to a nonspecific negative control antibody(TA50011),(Blue).
- Validation comment
- FC
