Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Other assay [1]
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Validation data
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- Product number
- PA5-23298 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RAP2A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse, Canine, Chicken/Avian, Xenopus
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1.0 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references Data in support of Rap2a GTPase expression, activation and effects in LPS-mediated innate immune response and NF-κB activation.
Carvalho BC, Oliveira LC, Rocha CD, Fernandes HB, Oliveira IM, Leãõ FB, Valverde TM, Rego IMG, Ghosh S, Silva AM
Data in brief 2019 Jun;24:103965
Data in brief 2019 Jun;24:103965
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAP2A in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide using a RAP2A polyclonal antibody (Product # PA5-23298) at 0.1 µg/mL. Goat anti-rabbit Ig HRP secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RAP2A in MCF7 cell lysate in the 1) absence and 2) presence of immunizing peptide. Samples were incubated in RAP2A polyclonal antibody (Product # PA5-23298) using a dilution of 0.1 µg/mL followed by a goat anti-rabbit Ig HRP secondary antibody. PicoTect ECL substrate solution was used for this test.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 2 Expression of GST-RBD-RalGDS and validation of affinity precipitation of Rap2a in mammalian cell extracts. ( a ) The expression of GST-RBD-RalGDS was obtained upon induction with 1 mM isopropyl-1-thio-beta- d -galactopyranoside (IPTG) for 2 hours of bacterial cell cultures transformed with pGEX-6P-1-RBD-RalGDS. Bacterial extracts were fractionated onto 10% SDS-PAGE, and followed by coomassie blue staining. ( b ) Coupling of GST-RBD-RalGDS to glutathione sepharose 4B (GS4B) beads. Suspensions of IPTG-induced bacterial cell cultures transformed with pGEX-6P-1 or pGEX-6P-1-RBD-RalGDS were centrifuged, lysed and sonicated. GST or GST-RBD-RalGDS fusion protein were mixed with GS4B. Bacterial cell lysates (lanes 1 and 2) and eluted samples (lanes 3-5) were separated by 10% SDS-PAGE, followed by coomassie blue staining. Two independent bacterial clones of pGEX-6P-1-RBD-RalGDS were used in lanes 4 and 5, respectively. ( c ) RAW264 cells were treated with PMA (100nM) as indicated. The lysates (500 mug) were then incubated with 100mul (~0.5mg) of bacterial lysates containing GST-RalGDS-RBD precoupled to GS4B beads. After washes, the beads mixtures were fractionated on 12% SDS-PAGE, transferred to PVDF membrane, and probed with anti-Rap2A antibody. Anti-Rabbit IgG (H + L), peroxidase conjugated was used as the secondary antibody. The detection was performed with Clarity Western ECL Blotting Substrate (BioRad) and followed by exposure to X-ray film. Densitometrical analysis of the