AHR-006-25UL

antibody from Invitrogen Antibodies

Targeting: HRH1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AHR-006-25UL

Provider

Invitrogen Antibodies

Product name

Histamine H1 Receptor/HRH1 (extracellular) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

25 µL

Concentration

0.8 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of human Jurkat T-cell leukemia cell line lysate (lanes 1 and 4), human U-87 MG glioblastoma cell line lysate (lanes 2 and 5) and human SH-SY5Y neuroblastoma cell line lysate (lanes 3 and 6): - 1-3. Anti- Histamine H1Receptor/HRH1 Antibody (extracellular) (#AHR-006), (1:200). 4-6. Anti-Histamine H1Receptor/HRH1 (extracellular) Antibody , preincubated with Histamine H1Receptor/HRH1 (extracellular) Blocking Peptide (#BLP-HR006).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of mouse brain membranes (lanes 1 and 5), rat brain membranes (lanes 2 and 6), rat lung membranes (lanes 3 and 7) and mouse lung membranes (lanes 4 and 8): - 1-4. Anti- Histamine H1Receptor/HRH1 (extracellular) Antibody (#AHR-006), (1:200). 5-8. Anti-Histamine H1Receptor/HRH1 (extracellular) Antibody , preincubated with Histamine H1Receptor/HRH1 (extracellular) Blocking Peptide (#BLP-HR006).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Expression of Histamine H1Receptor in mouse substantia nigra - Immunohistochemical staining of perfusion-fixed frozen mouse brain sections with Anti-Histamine H1 Receptor/HRH1 Antibody (extracellular) (#AHR-006), (1:300), followed by goat Anti-rabbit-AlexaFluor-488. A. Histamine H1Receptor immunoreactivity (green) appears in neuronal profiles (vertical arrows). B. Pre-incubation of the Antibody with Histamine H1Receptor (HRH1) (extracellular) Blocking Peptide (#BLP-HR006), suppresses staining. Cell nuclei are stained with DAPI (blue).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Cell surface detection of Histamine H1Receptor in live intact human MEG-01 megakaryoblastic leukemia cells: - (black line) cells. (red) Cells + goat- Anti-rabbit-FITC. (green) Cells + Anti-Histamine H1Receptor/HRH1 (extracellular) Antibody (#AHR-006), (5 µg) + goat- Anti-rabbit-FITC.