A300-596A

antibody from Invitrogen Antibodies

Targeting: MLLT3 AF-9, AF9, YEATS3

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A300-596A

Provider

Invitrogen Antibodies

Product name

AF9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

4° C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human AF9 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 10 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-AF9 antibody BL2311 (Cat. No. A300-596A) used for WB at 0.1 µg/ml (A) and 1 µg/ml (B), and used for IP at 3 µg/mg lysate. AF9 was also immunoprecipitated using rabbit anti-AF9 antibodies BL2310 (Cat. No. A300-595A), BL2312 and BL2313 (Cat. No. A300-597A) at 3 µg/mg lysate. For detecting immunoprecipitated AF9, ReliaBLOT™ reagents and procedures were used. Detection: Chemiluminescence with exposure times of 30 seconds (A and B).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection of human AF9 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-AF9 (Cat. No. A300-596A) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.