PA5-27797

antibody from Invitrogen Antibodies

Targeting: MLLT3 AF-9, AF9, YEATS3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27797

Provider

Invitrogen Antibodies

Product name

AF9 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, A431, HeLa, HepG2, THP-1, MLLT3 shRNA-transfected 293T, SEM-K2.

Concentration

0.46 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AF9 using 30 µg of THP-1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an AF9 polyclonal antibody (Product # PA5-27797) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AF9 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a AF9 Polyclonal Antibody (Product # PA5-27797) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using AF9 Polyclonal Antibody (Product # PA5-27797). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of AF9 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AF9 Polyclonal Antibody detects AF9 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. AF9 stained by AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded D54 xenograft, using AF9 (Product # PA5-27797) antibody at 1:750 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

AF9 Polyclonal Antibody detects AF9 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AF9 stained by AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.