Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-27797 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- AF9 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, THP-1, MLLT3 shRNA-transfected 293T, SEM-K2.
- Concentration
- 0.46 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of AF9 using 30 µg of THP-1 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an AF9 polyclonal antibody (Product # PA5-27797) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of AF9 was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a AF9 Polyclonal Antibody (Product # PA5-27797) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using AF9 Polyclonal Antibody (Product # PA5-27797). Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:3,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of AF9 was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:1000. Red: Phalloidin, a cytoskeleton marker. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AF9 Polyclonal Antibody detects AF9 protein at cytoplasm and nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. AF9 stained by AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded D54 xenograft, using AF9 (Product # PA5-27797) antibody at 1:750 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- AF9 Polyclonal Antibody detects AF9 protein at nucleus by immunohistochemical analysis. Sample: Paraffin-embedded mouse brain. AF9 stained by AF9 Polyclonal Antibody (Product # PA5-27797) diluted at 1:1500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.