AF6134

antibody from Novus Biologicals

Targeting: PRTN3 ACPA, AGP7, C-ANCA, MBT, P29, PR-3

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF6134

Provider

Novus Biologicals

Product name

Sheep Polyclonal Proteinase 3/Myeloblastin/PRTN3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) Cathepsin B and rhGranzyme B is observed.

Reactivity

Human

Host

Sheep

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Proteinase 3/Myeloblastin/ PRTN3 by Western Blot. Western blot shows lysates of HL-60 human acute promyelocytic leukemia cell line, human peripheral blood lymphocytes (PBL), and human peripheral blood mononuclear cells (PBMC). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Proteinase 3/Myeloblastin/ PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Simple Western^TM. Simple Western lane view shows lysates of human peripheral blood mononuclear cell tissue, loaded at 0.2 mg/mL. A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated) using 10 µg/mL of Sheep Anti-Human Proteinase 3/Myeloblastin/PRTN3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6134) followed by 1:50 dilution of HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.