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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [1]
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- Product number
- NBP1-25966 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-25966, RRID:AB_1726295
- Product name
- Rabbit Polyclonal Proteinase 3/Myeloblastin/PRTN3 Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- LYOPH
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Interaction between Tumor Cell Surface Receptor RAGE and Proteinase 3 Mediates Prostate Cancer Metastasis to Bone.
A pro-inflammatory role of C5L2 in C5a-primed neutrophils for ANCA-induced activation.
Kolonin MG, Sergeeva A, Staquicini DI, Smith TL, Tarleton CA, Molldrem JJ, Sidman RL, Marchiò S, Pasqualini R, Arap W
Cancer research 2017 Jun 15;77(12):3144-3150
Cancer research 2017 Jun 15;77(12):3144-3150
A pro-inflammatory role of C5L2 in C5a-primed neutrophils for ANCA-induced activation.
Hao J, Wang C, Yuan J, Chen M, Zhao MH
PloS one 2013;8(6):e66305
PloS one 2013;8(6):e66305
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Proteinase 3/Myeloblastin/PRTN3 Antibody [NBP1-25966] - Human PMN (peripheral blood mononuclear cells isolated from buffycoat; denatured, reduced) using Rabbit antibody to c-terminal region of Pr3 (Wegener autoantigen): whole serum at 1: 500 dilution; blocked with 1% LFDM for 15 minutes at room temperature with shake, primary antibody incubated for 15 minutes at room temperature, washed 3 times with PBST, 5 minutes each. Secondary antibody was also incubated for 15 minutes at room temperature.
