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- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- MAB6134 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Proteinase 3/Myeloblastin/PRTN3 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Proteinase 3/Myeloblastin/PRTN3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human Granzyme B is observed.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P24158- Isotype
- IgG
- Antibody clone number
- 684042
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references SERPINB1-mediated checkpoint of inflammatory caspase activation.
Choi YJ, Kim S, Choi Y, Nielsen TB, Yan J, Lu A, Ruan J, Lee HR, Wu H, Spellberg B, Jung JU
Nature immunology 2019 Mar;20(3):276-287
Nature immunology 2019 Mar;20(3):276-287
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Human Proteinase 3/Myeloblastin/PRTN3 by Western Blot. Western blot shows lysates of human granulocytes. PVDF Membrane was probed with 1 µg/mL of Human Proteinase 3/Myeloblastin/PRTN3 Mono-clonal Antibody (Catalog # MAB6134) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Proteinase 3/Myeloblastin/PRTN3 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
