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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-72500 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Complement C6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.36 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C6 in human blood plasma tissue lysate using 35 µg of protein per lane. Samples were probed with C6 polyclonal antibody (Product # PA5-72500) with a dilution of 1:1000, and a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of C6 in MDA-MB231 cells using confocal microscopy. Samples were treated with C6 H1 polyclonal antibody (Product # PA5-72500). Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) was used as a secondary antibody and DAPI was used to stain the cell nuclei (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of C6 in MDA-231 cells (bottom histogram) compared to a negative control cell (top histogram). Samples were incubated with C6 polyclonal antibody (Product # PA5-72500) followed by a FITC conjugated goat anti-rabbit secondary antibody.
