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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [2]
- Immunoprecipitation [1]
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- Product number
- NB100-1813 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-1813, RRID:AB_2251218
- Product name
- Rabbit Polyclonal NCAPG2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 0.2 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Condensin controls mitotic chromosome stiffness and stability without forming a structurally contiguous scaffold.
The condensin component NCAPG2 regulates microtubule-kinetochore attachment through recruitment of Polo-like kinase 1 to kinetochores.
Sun M, Biggs R, Hornick J, Marko JF
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2018 Dec;26(4):277-295
Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology 2018 Dec;26(4):277-295
The condensin component NCAPG2 regulates microtubule-kinetochore attachment through recruitment of Polo-like kinase 1 to kinetochores.
Kim JH, Shim J, Ji MJ, Jung Y, Bong SM, Jang YJ, Yoon EK, Lee SJ, Kim KG, Kim YH, Lee C, Lee BI, Kim KT
Nature communications 2014 Aug 11;5:4588
Nature communications 2014 Aug 11;5:4588
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCAPG2 Antibody [NB100-1813] - Detection of Human CAP-G2 on HeLa whole cell lysate using NB100-1813. CAP-G2 was also IPed by a rabbit anti-CAP-G2 antibody, and CAP-G2 was co-immunoprecipitated by a rabbit anti-CAP-D3 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NCAPG2 Antibody [NB100-1813] - Detection of Human CAP-G2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-G2 antibody NB100-1813 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: NCAPG2 Antibody [NB100-1813] - Detection of human CAP-G2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-CAP-G2 antibody NB100-1813 used for IP at 6 ug per reaction. CAP-G2 was also immunoprecipitated by another rabbit anti-CAP-G2 antibody. For blotting immunoprecipitated CAP-G2, NB100-1813 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
