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LearnNB100-56166
antibody from Novus Biologicals
Targeting: DIABLO
DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-56166 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56166, RRID:AB_838882
- Product name
- Rabbit Polyclonal SMAC/Diablo Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. Smac
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Analysis of apoptosis protein expression in early-stage colorectal cancer suggests opportunities for new prognostic biomarkers.
Krajewska M, Kim H, Kim C, Kang H, Welsh K, Matsuzawa S, Tsukamoto M, Thomas RG, Assa-Munt N, Piao Z, Suzuki K, Perucho M, Krajewski S, Reed JC
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Aug 1;11(15):5451-61
Clinical cancer research : an official journal of the American Association for Cancer Research 2005 Aug 1;11(15):5451-61
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56166] - Analysis of Smac/DIABLO human tissues and cell lines using this antibody. Legend. SMAC: full-length recombinant smac. RS1846 and Jurkat are tumor cell lines. All tissues are from normal donors except colon carcinoma (Colon Ca). SMAC expression was detected in all samples except spleen and normal colon.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMAC/Diablo Antibody - Unpurified [NB100-56166] - Analysis of A) MCF7 and B) K562 cell lysate using SMAC/Diablo this antibody at 1:500.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56166] - Formalin-fixed, paraffin-embedded human colon stained for Smac/DIABLO using this antibody at 1:2000. A) normal colon. B) and C) colon carcinoma. Only trace Smac/DIABLO immunoreactivity is seen in normal colonic epithelium A), which is seen in the apical, foveolar cells. In contrast, strong immunoreactivity is observed in B) nuclear and C) cytoplasmic locations in the colon carcinoma specimen. Hematoxylin-eosin counterstain.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SMAC/Diablo Antibody - Unpurified [NB100-56166] - Formalin-fixed, paraffin-embedded tissue section of human plasmacytoma (a mature B cell lymphoma) stained for Smac using this antibody at 1:2000. Intense nuclear staining is observed. Hematoxylin-eosin counterstain.
