A21983

antibody from Invitrogen Antibodies

Targeting: DIABLO DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC

Immunocytochemistry (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

A21983

Provider

Invitrogen Antibodies

Product name

Anti-DIABLO Monoclonal Antibody (8H5AA3)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

8H5AA3

Vial size

100 µg

Concentration

1 mg/mL

Storage

4° C

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Human HDFn cells were paraformaldehyde fixed (4%, 20 min) and Triton X-100 permeabilized (0.1%, 15 min). The cells were incubated with the antibody at 1 µg/mL overnight at 4°C. Secondary antibody: (red) Alexa Fluor® 594 goat anti-mouse IgG (H+L) at a 1/1000 dilution for 1h. 10% Goat serum was used as the blocking agent for all blocking steps. DAPI was used to stain the cell nuclei (blue). Target protein locates mainly in mitochondria.