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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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- Product number
- TA319299 - Provider product page
- Provider
- OriGene
- Product name
- Rabbit polyclonal Smac/Diablo antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal Smac/Diablo antibody
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- DIABLO
- Isotype
- IgG
- Antibody clone number
- NULL
- Vial size
- 100 µl
- Concentration
- 85 mg/mL
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- WB using anti-Smac detects a 26 kDa band when 1 ug of recombinant Smac is applied (lane 1). Lane 2 shows Smac detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied. Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 ?M etoposide. Recombinant Smac migrates slower than the native form because of the His6-tag. The primary antibody was used at 1:1000. HRP Goat-a-Rabbit antibody was 1:1000.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by western blot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1. Other detection systems will yield similar results.
- Validation comment
- WB
