Explore
Validate
LearnPAB11261
antibody from Abnova Corporation
Targeting: DIABLO
DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB11261 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB11261, RRID:AB_1673702
- Product name
- DIABLO polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against partial recombinant DIABLO.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Molecular determinants of the caspase-promoting activity of Smac/DIABLO and its role in the death receptor pathway.
Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition.
Structural basis of IAP recognition by Smac/DIABLO.
Srinivasula SM, Datta P, Fan XJ, Fernandes-Alnemri T, Huang Z, Alnemri ES
The Journal of biological chemistry 2000 Nov 17;275(46):36152-7
The Journal of biological chemistry 2000 Nov 17;275(46):36152-7
Smac, a mitochondrial protein that promotes cytochrome c-dependent caspase activation by eliminating IAP inhibition.
Du C, Fang M, Li Y, Li L, Wang X
Cell 2000 Jul 7;102(1):33-42
Cell 2000 Jul 7;102(1):33-42
Structural basis of IAP recognition by Smac/DIABLO.
Wu G, Chai J, Suber TL, Wu JW, Du C, Wang X, Shi Y
Nature 2000 Dec 21-28;408(6815):1008-12
Nature 2000 Dec 21-28;408(6815):1008-12
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DIABLO polyclonal antibody (Cat # PAB11261) is shown to detect a 25-26 kDa band in partially purified recombinant human DIABLO protein by immunoblot.Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively.The blot was incubated overnight with a 1 : 1000 dilution of DIABLO polyclonal antibody in TBST.Detection occurs using a 1 : 1000 dilution of HRP Goat-a-Rabbit with visualization via ECL.Film exposure approximately 1'.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblot using DIABLO polyclonal antibody (Cat # PAB11261) detects a 26 kDa band when 1 ug of recombinant DIABLO is applied (lane 1).Lane 2 shows DIABLO detection when 30 ug of 1% NP-40 treated cell lysate from HeLa cells is applied.Lanes 3 & 4 show 30 ug each of cytosolic fractions from HeLa cell lysates both with (lane 3) and without (lane 4) treatment with 30 micro;M etoposide.Recombinant DIABLO migrates slower than the native form because of the His6-tag.The blot was incubated overnight with a 1:1000 dilution of DIABLO polyclonal antibody.
