F54888

antibody from NSJ Bioreagents

Targeting: DIABLO DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54888

Provider

NSJ Bioreagents

Product name

SMAC/Diablo Antibody

Antibody type

Polyclonal

Description

This highly specific SMAC/Diablo antibody is suitable for use in Flow cytometry/Immunohistochemistry/Western blot applications with human samples.

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the SMAC/Diablo antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human 1) HeLa and 2) Jurkat cell lysate with SMAC/Diablo antibody. Predicted molecular weight: 21-27 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human T-47D cell lysate with SMAC/Diablo antibody. Predicted molecular weight: 21-27 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human testis tissue with SMAC/Diablo antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human MDA-MB-231 cells with SMAC/Diablo antibody; Blue=isotype control, Green= SMAC/Diablo antibody.