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LearnAP09077SU-N
antibody from Acris Antibodies GmbH
Targeting: DIABLO
DFNA64, DIABLO-S, FLJ10537, FLJ25049, SMAC
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- AP09077SU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09077SU-N, RRID:AB_2035962
- Product name
- anti DIABLO / SMAC (56-239)
- Antibody type
- Polyclonal
- Antigen
- Recombinant His6-tagged human Smac/DIABLO protein (amino acids 56-239)
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 85 mg/ml (by Refractometry)
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Anti-Smac is shown to detect a 25-26 kDa band in partially purified recombinant human Smac protein by immunoblot. Lanes 1-3 are loaded with 1, 10 and 100 ng of protein per lane, respectively. The blot was incubated overnight with a 1:1000 dilution of anti-Smac in TBST. Detection occurs using a 1:1000 dilution of HRP Goat-a-Rabbit with visualization via ECL. Film exposure approximately 1'. Other detection systems will yield similar results.
