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- Antigen structure
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- Product number
- IC30402A - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IL-32 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human IL-32 alpha and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 30% cross-reactivity with recombinant human (rh) IL-32 beta and 38% cross-reactivity with rhIL-32 gamma is detected.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references Role of Interleukin 32 in Human Immunodeficiency Virus Reactivation and Its Link to Human Immunodeficiency Virus-Herpes Simplex Virus Coinfection.
Dendritic Cell-Derived IL-32α: A Novel Inhibitory Cytokine of NK Cell Function.
NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
Mesquita PMM, Preston-Hurlburt P, Keller MJ, Vudattu N, Espinoza L, Altrich M, Anastos K, Herold KC, Herold BC
The Journal of infectious diseases 2017 Feb 15;215(4):614-622
The Journal of infectious diseases 2017 Feb 15;215(4):614-622
Dendritic Cell-Derived IL-32α: A Novel Inhibitory Cytokine of NK Cell Function.
Gorvel L, Korenfeld D, Tung T, Klechevsky E
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1290-1300
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1290-1300
NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.
Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
PloS one 2015;10(8):e0134568
PloS one 2015;10(8):e0134568
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Detection of IL-32 alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL-32 alpha APC-conjugated Monoclonal Antibody (Catalog # IC30402A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
