PAB16923

antibody from Abnova Corporation

Targeting: IL32 NK4, TAIF, TAIFb, TAIFd

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB16923

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB16923, RRID:AB_10678030

Product name

IL32 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against full length recombinant IL32.

Storage

Store at 4°C on dry atmosphere.After reconstitution with 0.1 mL of deionized water, store at -20°C or lower.Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Molecular characterization of IL-32 in human endothelial cells.

Kobayashi H, Lin PC
Cytokine 2009 Jun;46(3):351-8

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Interleukin-32 expression in the pancreas.

Nishida A, Andoh A, Inatomi O, Fujiyama Y
The Journal of biological chemistry 2009 Jun 26;284(26):17868-76

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Negative feedback regulation of IL-32 production by iNOS activation in response to dsRNA or influenza virus infection.

Li W, Yang F, Liu Y, Gong R, Liu L, Feng Y, Hu P, Sun W, Hao Q, Kang L, Wu J, Zhu Y
European journal of immunology 2009 Apr;39(4):1019-24

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot using IL32 polyclonal antibody (Cat # PAB16923) shows detection of a band ~19 KDa in size corresponding to recombinant human IL32. After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1 : 1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG secondary antibody diluted 1 : 40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax™ chemiluminescent substrate. Image was captured using VersaDoc™ MP 4000 imaging system (Bio-Rad).