Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-76684 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76684, RRID:AB_11006197
- Product name
- Rabbit Polyclonal IL-32 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. This antibody detects all isoforms of IL-32.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Novel Immunoregulatory Functions of IL-18, an Accomplice of TGF-β1.
A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa.
Interleukin-32 is highly expressed in lesions of hidradenitis suppurativa.
Casu B, Dondero A, Regis S, Caliendo F, Petretto A, Bartolucci M, Bellora F, Bottino C, Castriconi R
Cancers 2019 Jan 11;11(1)
Cancers 2019 Jan 11;11(1)
A systematic review and critical evaluation of immunohistochemical associations in hidradenitis suppurativa.
Frew JW, Hawkes JE, Krueger JG
F1000Research 2018;7:1923
F1000Research 2018;7:1923
Interleukin-32 is highly expressed in lesions of hidradenitis suppurativa.
Thomi R, Yerly D, Yawalkar N, Simon D, Schlapbach C, Hunger RE
The British journal of dermatology 2017 Nov;177(5):1358-1366
The British journal of dermatology 2017 Nov;177(5):1358-1366
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-32 Antibody [NBP1-76684] - Human spleen cell lysate with IL-32 antibody at (A) 5 and (B) 10 ug/ml shows two isoforms of IL-32.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-32 Antibody [NBP1-76684] - Western Blot Validation in Human Spleen. Lysate, showing two isoforms of IL-32 Loading: 15 ug of lysates per lane. Antibodies: IL-32, NBP1-76684 (lane 1: 2 ug/mL and lane 2: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IL-32 Antibody [NBP1-76684] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human IL-32 recombinant protein per lane. Antibodies: IL-32, NBP1-76684 (Lane 1: 0.5 ug/ml, Lane 2: 1 ug/ml, Lane 3: 2 ug/ml and Lane 4: 4 ug/ml), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 17 kD.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-76684] - Human spleen tissue with IL-32 antibody at 10 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IL-32 Antibody [NBP1-76684] - Mouse spleen tissue with IL 32 antibody at 2 ug/mL.