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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- AP09192PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AP09192PU-N, RRID:AB_2035689
- Product name
- anti Interleukin-32 / IL32 (Isoform 4 / alpha)
- Antibody type
- Polyclonal
- Antigen
- Full length recombinant Human IL-32A protein
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/mL by UV absorbance at 280 nm
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Supportive validation
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- Acris Antibodies GmbH (provider)
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- Experimental details
- Western blot using anti-Human IL-32A antibody shows detection of a band ~19 kDa in size corresponding to recombinant Human IL-32A (Lane 1). The identity of the higher molecular weight band is unknown. Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1/1,000 dilution. Detection occurred using peroxidase conjugated anti-Rabbit IgG (Cat.-No R1454HRP) secondary antibody diluted 1/40,000 in blocking buffer for 30 min at RT followed by reaction with FemtoMax⢠chemiluminescent substrate. Image was captured using VersaDoc⢠MP 4000 imaging system (Bio-Rad).
