IC30402A

antibody from R&D Systems

Targeting: IL32 NK4, TAIF, TAIFb, TAIFd

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

IC30402A

Provider

R&D Systems

Product name

Human IL-32 APC-conjugated Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human IL-32 alpha and other IL-32 isoforms in direct ELISAs. In direct ELISAs, 30% cross-reactivity with recombinant human (rh) IL-32 beta and 38% cross-reactivity with rhIL-32 gamma is detected.

Reactivity

Human

Host

Rat

Conjugate

Red dye

Antigen sequence

P24001


Isotype

IgG

Antibody clone number

373821

Vial size

100 Tests

Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

References

Submitted references

Role of Interleukin 32 in Human Immunodeficiency Virus Reactivation and Its Link to Human Immunodeficiency Virus-Herpes Simplex Virus Coinfection.

Mesquita PMM, Preston-Hurlburt P, Keller MJ, Vudattu N, Espinoza L, Altrich M, Anastos K, Herold KC, Herold BC
The Journal of infectious diseases 2017 Feb 15;215(4):614-622

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Dendritic Cell-Derived IL-32α: A Novel Inhibitory Cytokine of NK Cell Function.

Gorvel L, Korenfeld D, Tung T, Klechevsky E
Journal of immunology (Baltimore, Md. : 1950) 2017 Aug 15;199(4):1290-1300

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NKP30-B7-H6 Interaction Aggravates Hepatocyte Damage through Up-Regulation of Interleukin-32 Expression in Hepatitis B Virus-Related Acute-On-Chronic Liver Failure.

Zou Y, Bao J, Pan X, Lu Y, Liao S, Wang X, Wang G, Lin D
PloS one 2015;10(8):e0134568

Flow cytometry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of IL-32 alpha in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with PMA and calcium ionomycin were stained with Rat Anti-Human IL-32 alpha APC-conjugated Monoclonal Antibody (Catalog # IC30402A, filled histogram) or isotype control antibody (Catalog # IC006A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.