Antibody data
- Antibody Data
- Antigen structure
- References [5]
- Comments [0]
- Validations
- Western blot [3]
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Validation data
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- Product number
- NB100-534 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-534, RRID:AB_2210886
- Product name
- Rabbit Polyclonal Aprataxin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. NB100-534 is specific for human Aprataxin protein.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Genomic maps of long noncoding RNA occupancy reveal principles of RNA-chromatin interactions.
Aprataxin localizes to mitochondria and preserves mitochondrial function.
Protein kinase C gamma, a protein causative for dominant ataxia, negatively regulates nuclear import of recessive-ataxia-related aprataxin.
Nuclear ataxia-telangiectasia mutated (ATM) mediates the cellular response to DNA double strand breaks in human neuron-like cells.
A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment.
Chu C, Qu K, Zhong FL, Artandi SE, Chang HY
Molecular cell 2011 Nov 18;44(4):667-78
Molecular cell 2011 Nov 18;44(4):667-78
Aprataxin localizes to mitochondria and preserves mitochondrial function.
Sykora P, Croteau DL, Bohr VA, Wilson DM 3rd
Proceedings of the National Academy of Sciences of the United States of America 2011 May 3;108(18):7437-42
Proceedings of the National Academy of Sciences of the United States of America 2011 May 3;108(18):7437-42
Protein kinase C gamma, a protein causative for dominant ataxia, negatively regulates nuclear import of recessive-ataxia-related aprataxin.
Asai H, Hirano M, Shimada K, Kiriyama T, Furiya Y, Ikeda M, Iwamoto T, Mori T, Nishinaka K, Konishi N, Udaka F, Ueno S
Human molecular genetics 2009 Oct 1;18(19):3533-43
Human molecular genetics 2009 Oct 1;18(19):3533-43
Nuclear ataxia-telangiectasia mutated (ATM) mediates the cellular response to DNA double strand breaks in human neuron-like cells.
Biton S, Dar I, Mittelman L, Pereg Y, Barzilai A, Shiloh Y
The Journal of biological chemistry 2006 Jun 23;281(25):17482-91
The Journal of biological chemistry 2006 Jun 23;281(25):17482-91
A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment.
Luo H, Chan DW, Yang T, Rodriguez M, Chen BP, Leng M, Mu JJ, Chen D, Songyang Z, Wang Y, Qin J
Molecular and cellular biology 2004 Oct;24(19):8356-65
Molecular and cellular biology 2004 Oct;24(19):8356-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aprataxin Antibody [NB100-534] - Detection of Human Aprataxin by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody NB100-534 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aprataxin Antibody [NB100-534] - Whole cell lysate (50 ug) from normal human lymphoblasts (C3ABR cells; WT) or lymphoblasts from an AOA patient (738RM cells; AOA). Antibody used at 0.1 and 0.04 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Aprataxin Antibody [NB100-534] - Detection of human Aprataxin by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Aprataxin antibody NB100-534 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.