- UM800074 - OriGene RTRAF antibody

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Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot analysis of extracts (15ug) from 9 Human tissue by using anti-C14orf166 monoclonal antibody (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: colon:;9:Spleen).(1:500) Dilution: 1:500
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Western blot of cell lysates (35ug) from 9 different cell lines (1: HepG2, 2: HeLa, 3: SV-T2, 4: A549, 5: COS7, 6: Jurkat, 7: MDCK, 8: PC-12, 9: MCF7). Diluation: 1:250
Validation comment: WB

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunofluorescent staining of HeLa cells using anti-C14orf166 mouse monoclonal antibody (UM800074, green, 1:50). Actin filaments were labeled with Alexa Fluor? 594 Phalloidin (red), and nuclear with DAPI (blue).
Validation comment: IF

Supportive validation

Submitted by: OriGene (provider)
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Experimental details: Immunohistochemical staining of paraffin-embedded Human melanoma tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800074)(1:200)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800074)(1:200)
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800074)(1:200)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum using anti-C14orf166 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800074)(1:200)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human embryonic brain cortex tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110 ¡C for 10 min, UM800074)(1:200)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded Human adult heart tissue using anti-C14orf166 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 110C for 10 min, UM800074)(1:200)

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human colon cancer using anti-C14ORF166 clone UMAB182 mouse monoclonal antibody at 1:600 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800074 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells have strong nuclear and weak cytoplasmic staining.
Validation comment: IHC

Supportive validation

Submitted by: OriGene (provider)
Main image
Experimental details: Immunohistochemical staining of paraffin-embedded human prostate cancer using anti-C14ORF166 clone UMAB182 mouse monoclonal antibody at 1:600 dilution of 1mg/mL and detection with Polink2 Broad HRP DAB. UM800074 requires heat-induced epitope retrieval with Accel for 3minutes at110C in pressure chamber . The image shows the tumor cells have strong nuclear and intermediate levels of cytoplasmic staining.
Validation comment: IHC

UM800074