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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- MAB6760 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal Nidogen-2 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse Nidogen-2 in direct ELISAs. In direct ELISAs, approximately 80% cross-reactivity with recombinant human (rh) Nidogen-2 and no cross-reactivity with rhNidogen-1 is observed.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse Nidogen-2 by Western Blot. Western blot shows lysates of mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Rat Anti-Mouse Nidogen-2 Monoclonal Antibody (Catalog # MAB6760) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Nidogen-2 at approximately 120-200 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
