Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Nidogen 2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human Nidogen-1 is observed. Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunohistochemical analysis of Nidogen 2 in immersion fixed paraffin-embedded sections of normal human kidney. Samples were incubated in Nidogen 2 polyclonal antibody (Product # PA5-47482) using a dilution of 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific staining was localized to epithelial cells in convoluted tubules.