AP02275SU-S

antibody from Acris Antibodies GmbH

Targeting: NID2

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Radioimmunoassay (Recommended by provider)

Antibody Data

Product number

AP02275SU-S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#AP02275SU-S, RRID:AB_1620178

Product name

anti Nidogen-2

Antibody type

Polyclonal

Antigen

Recombinant Mouse Nidogen 2

Reactivity

Mouse

Host

Rabbit

Vial size

20 µl

References

Submitted references

The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens.

Behrens DT, Villone D, Koch M, Brunner G, Sorokin L, Robenek H, Bruckner-Tuderman L, Bruckner P, Hansen U
The Journal of biological chemistry 2012 May 25;287(22):18700-9

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Deposition of nidogens and other basement membrane proteins in the young and aging mouse retina.

Kunze A, Abari E, Semkova I, Paulsson M, Hartmann U
Ophthalmic research 2010;43(2):108-12

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Gene structure and functional analysis of the mouse nidogen-2 gene: nidogen-2 is not essential for basement membrane formation in mice.

Schymeinsky J, Nedbal S, Miosge N, Pöschl E, Rao C, Beier DR, Skarnes WC, Timpl R, Bader BL
Molecular and cellular biology 2002 Oct;22(19):6820-30

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Binding of mouse nidogen-2 to basement membrane components and cells and its expression in embryonic and adult tissues suggest complementary functions of the two nidogens.

Salmivirta K, Talts JF, Olsson M, Sasaki T, Timpl R, Ekblom P
Experimental cell research 2002 Oct 1;279(2):188-201

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Nidogen-2: a new basement membrane protein with diverse binding properties.

Kohfeldt E, Sasaki T, Göhring W, Timpl R
Journal of molecular biology 1998 Sep 11;282(1):99-109

Immunohistochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Immunofluorescence of Nidogen 2 staining in Paraffin sections of cardiac muscle tissue from control (+/+) and Nidogen 2-mutant (-/-) mice. Image B: In control sections, staining for Nidogen 2 was prominent around capillaries, but not around cardiomyocytes. Image E: In Sections of mutant heart, not specific Nidogen 2-staining was observed. Schymeinsky J et al. (2002) Mol Cell Biol 22(19): 6820-6830.