AF1008
antibody from Novus Biologicals
Targeting: LTBR
D12S370, TNF-R-III, TNFCR, TNFR-RP, TNFR2-RP, TNFRSF3
Antibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- AF1008 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal Lymphotoxin beta R/TNFRSF3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse Lymphotoxin beta R/TNFRSF3 in direct ELISA and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human (rh) LT beta R is observed, and less than 1% cross-reactivity with recombinant mouse (rm) 4-1BB, rmCD27, rmOPG, rmRANK, rmCD30, rmCD40, rhDR6, rmTNF RI, rmTNF RII, rmEDAR, rmFas and rmGITR is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Fibroblast reticular cells engineer a blastema extracellular network during digit tip regeneration in mice.
A dendritic-cell-stromal axis maintains immune responses in lymph nodes.
Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex.
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
CXCL13 production by an established lymph node stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways.
Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes.
Marrero L, Simkin J, Sammarco M, Muneoka K
Regeneration (Oxford, England) 2017 Apr;4(2):69-84
Regeneration (Oxford, England) 2017 Apr;4(2):69-84
A dendritic-cell-stromal axis maintains immune responses in lymph nodes.
Kumar V, Dasoveanu DC, Chyou S, Tzeng TC, Rozo C, Liang Y, Stohl W, Fu YX, Ruddle NH, Lu TT
Immunity 2015 Apr 21;42(4):719-30
Immunity 2015 Apr 21;42(4):719-30
Autotaxin produced by stromal cells promotes LFA-1-independent and Rho-dependent interstitial T cell motility in the lymph node paracortex.
Katakai T, Kondo N, Ueda Y, Kinashi T
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):617-26
Journal of immunology (Baltimore, Md. : 1950) 2014 Jul 15;193(2):617-26
TNF-alpha drives remodeling of blood vessels and lymphatics in sustained airway inflammation in mice.
Baluk P, Yao LC, Feng J, Romano T, Jung SS, Schreiter JL, Yan L, Shealy DJ, McDonald DM
The Journal of clinical investigation 2009 Oct;119(10):2954-64
The Journal of clinical investigation 2009 Oct;119(10):2954-64
CXCL13 production by an established lymph node stromal cell line via lymphotoxin-beta receptor engagement involves the cooperation of multiple signaling pathways.
Suto H, Katakai T, Sugai M, Kinashi T, Shimizu A
International immunology 2009 Apr;21(4):467-76
International immunology 2009 Apr;21(4):467-76
Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6189-200
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6189-200
Organizer-like reticular stromal cell layer common to adult secondary lymphoid organs.
Katakai T, Suto H, Sugai M, Gonda H, Togawa A, Suematsu S, Ebisuno Y, Katagiri K, Kinashi T, Shimizu A
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6189-200
Journal of immunology (Baltimore, Md. : 1950) 2008 Nov 1;181(9):6189-200
Lymph node fibroblastic reticular cells construct the stromal reticulum via contact with lymphocytes.
Katakai T, Hara T, Sugai M, Gonda H, Shimizu A
The Journal of experimental medicine 2004 Sep 20;200(6):783-95
The Journal of experimental medicine 2004 Sep 20;200(6):783-95
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Lymphotoxin beta R/TNFRSF3 in NIH-3T3 Mouse Cell Line by Flow Cytometry. NIH-3T3 mouse embryonic fibroblast cell line was stained with Goat Anti-Mouse Lymphotoxin beta R/TNFRSF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1008, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107). View our protocol for Staining Membrane-associated Proteins.