PAB28697

antibody from Abnova Corporation

Targeting: S100A12 CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB28697

Provider

Abnova Corporation

Product name

S100A12 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant S100A12.

Antigen sequence

KLEEHLEGIVNIFHQYSVRKGHFDTLSKGELKQLL
TKELANTIKNIKDKAVIDEIFQGLDANQDEQVDFQ
EFISLVAIALKAAHYHTHKE


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot anyalysis of Lane 1: Negative control (vector only transfected HEK293T lysate), Lane 2: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY401723) with S100A12 polyclonal antibody (Cat # PAB28697) at 1:100-1:250 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 with S100A12 polyclonal antibody (Cat # PAB28697) at 1-4 ug/mL shows positivity in nucleus but not nucleoli.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human bone marrow with S100A12 polyclonal antibody (Cat # PAB28697) shows strong nuclear positivity in bone marrow poietic cells at 1:500-1:1000 dilution.

Validation comment

Immunohistochemistry