BAF1052

antibody from Novus Biologicals

Targeting: S100A12 CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

BAF1052

Provider

Novus Biologicals

Product name

Goat Polyclonal EN-RAGE/S100A12 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects EN-RAGE/S100A12 in Western blots.

Reactivity

Human

Host

Goat

Conjugate

Biotin

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

S100/Calgranulin-mediated inflammation accelerates left ventricular hypertrophy and aortic valve sclerosis in chronic kidney disease in a receptor for advanced glycation end products-dependent manner.

Yan L, Mathew L, Chellan B, Gardner B, Earley J, Puri TS, Hofmann Bowman MA
Arteriosclerosis, thrombosis, and vascular biology 2014 Jul;34(7):1399-411

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Plasma profiles in active systemic juvenile idiopathic arthritis: Biomarkers and biological implications.

Ling XB, Park JL, Carroll T, Nguyen KD, Lau K, Macaubas C, Chen E, Lee T, Sandborg C, Milojevic D, Kanegaye JT, Gao S, Burns J, Schilling J, Mellins ED
Proteomics 2010 Dec;10(24):4415-30

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

EN-RAGE/S100A12 in Human Tonsil. EN-RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human EN-RAGE/S100A12 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1052) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

EN-RAGE/S100A12 in Human Tonsil. EN-RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 10 µg/mL Goat Anti-Human EN-RAGE/ S100A12 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.