PA5-47056
antibody from Invitrogen Antibodies
Targeting: S100A12
CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-47056 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- S100A12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-human EN-RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for EN-RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A12 in human granulocytes. Samples were incubated in S100A12 polyclonal antibody (Product # PA5-47056) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for EN‚RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of S100A12 in 0.2 mg/mL lysates of human tonsil. Samples were incubated in S100A12 polyclonal antibody (Product # PA5-47056) at a dilution of 10 µg/mL. A specific band was detected for EN-RAGE/S100A12 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of S100A12 in immersion fixed paraffin-embedded sections of human tonsil. Samples were incubated in S100A12 polyclonal antibody (Product # PA5-47056) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).