PA5-47056

antibody from Invitrogen Antibodies

Targeting: S100A12 CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Antibody Data

Product number

PA5-47056

Provider

Invitrogen Antibodies

Product name

S100A12 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Reconstitute at 0.2 mg/mL in sterile PBS.

Reactivity

Human

Host

Goat

Isotype

IgG

Vial size

100 µg

Concentration

0.2 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis from lysate of human granulocytes. PVDF membrane was probed with 1 µg/mL of Goat Anti-human EN-RAGE/S100A12 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47056) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for EN-RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of S100A12 in human granulocytes. Samples were incubated in S100A12 polyclonal antibody (Product # PA5-47056) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for EN‚RAGE/S100A12 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of S100A12 in 0.2 mg/mL lysates of human tonsil. Samples were incubated in S100A12 polyclonal antibody (Product # PA5-47056) at a dilution of 10 µg/mL. A specific band was detected for EN-RAGE/S100A12 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of S100A12 in immersion fixed paraffin-embedded sections of human tonsil. Samples were incubated in S100A12 polyclonal antibody (Product # PA5-47056) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).