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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [7]
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- Product number
- NBP1-86694 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86694, RRID:AB_11038560
- Product name
- Rabbit Polyclonal EN-RAGE/S100A12 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human EN-RAGE/S100A12 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EN-RAGE/S100A12 Antibody [NBP1-86694] - Analysis in human spleen tissue.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EN-RAGE/S100A12 Antibody [NBP1-86694] - Analysis in control (vector only transfected HEK293T lysate) and S100A12 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EN-RAGE/S100A12 Antibody [NBP1-86694] - Staining of human bone marrow shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EN-RAGE/S100A12 Antibody [NBP1-86694] - Staining of human cerebral cortex shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EN-RAGE/S100A12 Antibody [NBP1-86694] - Staining of human bone marrow shows strong cytoplasmic and nuclear positivity in hematopoietic cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EN-RAGE/S100A12 Antibody [NBP1-86694] - Staining of human cerebral cortex shows no positivity in neurons as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EN-RAGE/S100A12 Antibody [NBP1-86694] - Staining of human skin shows no positivity in squamous epithelial cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EN-RAGE/S100A12 Antibody [NBP1-86694] - Staining of human testis shows no positivity in cells in seminiferous ducts as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: EN-RAGE/S100A12 Antibody [NBP1-86694] - Staining in human bone marrow and testis tissues using NBP1-86694 antibody. Corresponding S100A12 RNA-seq data are presented for the same tissues.
