Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Immunohistochemistry [2]
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Validation data
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- Product number
- BAF1052 - Provider product page
- Provider
- R&D Systems
- Product name
- Human EN-RAGE/S100A12 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects EN-RAGE/S100A12 in Western blots.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Antigen sequence
P80511
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references S100/Calgranulin-mediated inflammation accelerates left ventricular hypertrophy and aortic valve sclerosis in chronic kidney disease in a receptor for advanced glycation end products-dependent manner.
Plasma profiles in active systemic juvenile idiopathic arthritis: Biomarkers and biological implications.
Yan L, Mathew L, Chellan B, Gardner B, Earley J, Puri TS, Hofmann Bowman MA
Arteriosclerosis, thrombosis, and vascular biology 2014 Jul;34(7):1399-411
Arteriosclerosis, thrombosis, and vascular biology 2014 Jul;34(7):1399-411
Plasma profiles in active systemic juvenile idiopathic arthritis: Biomarkers and biological implications.
Ling XB, Park JL, Carroll T, Nguyen KD, Lau K, Macaubas C, Chen E, Lee T, Sandborg C, Milojevic D, Kanegaye JT, Gao S, Burns J, Schilling J, Mellins ED
Proteomics 2010 Dec;10(24):4415-30
Proteomics 2010 Dec;10(24):4415-30
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EN-RAGE/S100A12 in Human Tonsil. EN-RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using 10 µg/mL Goat Anti-Human EN-RAGE/ S100A12 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1052) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- EN-RAGE/S100A12 in Human Tonsil. EN-RAGE/S100A12 was detected in immersion fixed paraffin-embedded sections of human tonsil using Goat Anti-Human EN-RAGE/S100A12 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1052) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.