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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
- Flow cytometry [3]
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- Product number
- TA502750 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA502750, RRID:AB_11126790
- Product name
- PRKAR1B (PKA regulatory subunit I beta) mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)
- Antibody type
- Monoclonal
- Description
- PRKAR1B (PKA regulatory subunit I beta) mouse monoclonal antibody, clone OTI2A3 (formerly 2A3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- PRKAR1B
- Isotype
- IgG
- Antibody clone number
- OTI2A3
- Vial size
- 100 µl
- Concentration
- 0.57 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY PRKAR1B (RC207809 , Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PRKAR1B.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-PRKAR1B mouse monoclonal antibody (TA502750) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY PRKAR1B(RC207809 ).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502750)
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-PRKAR1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502750)
- Validation comment
- IHC
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Jurkat cells, using anti-PRKAR1B antibody(TA502750),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC207809 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-PRKAR1B antibody(TA502750), and then analyzed by flow cytometry.
- Validation comment
- FC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Flow cytometric Analysis of Hela cells, using anti-PRKAR1B antibody(TA502750),(Red), compared to a nonspecific negative control antibody,(Blue).
- Validation comment
- FC
