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Western blot
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
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- Product number
- MA5-25526 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PRKAR1B Monoclonal Antibody (OTI9C5)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- OTI9C5
- Vial size
- 100 µL
- Concentration
- 0.17 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRKAR1B in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PRKAR1B (Product # MA5-25526) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRKAR1B in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PRKAR1B (Product # MA5-25526) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PRKAR1B in HEK293T cells in untransfected (Left lane) and transfected (Right lane) samples using 5 µg per lane. The samples were separated by SDS-PAGE and probed with PRKAR1B (Product # MA5-25526) monoclonal antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using anti-PRKAR1B Monoclonal Antibody (OTI9C5) (Product # MA5-25526) and a 55 kDa band corresponding to PRKAR1B was observed across tissues tested. Higher expression of PRKAR1B was observed in Mouse lung, Rat lung as compared to Mouse skeletal muscle and Rat skeletal muscle. Whole cell extracts (30 µg lysate) of Mouse skeletal muscle (Lane 1), Rat skeletal muscle (Lane 2), Mouse lung (Lane 3), Rat lung (Lane 4) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PRKAR1B in COS7 cells. Cells were transfected with a plasmid overexpressing PRKAR1B and probed with a PRKAR1B monoclonal antibody (Product # MA5-25526).
