AMAb90789

antibody from Atlas Antibodies

Targeting: ZNF703 FLJ14299, NLZ1, ZEPPO1, ZNF503L, Zpo1

Antibody data

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Validation data

Product number: AMAb90789 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#AMAb90789, RRID:AB_2665667
Product name: Anti-ZNF703
Antibody type: Monoclonal
Reactivity: Human
Host: Mouse
Conjugate: Unconjugated
Antigen sequence: PYSKGSGGGDSRKDSGSSSVSSTSSSSSSSPGDKA GFRVPSAACPPFPPHGAPVSASSSSS
Epitope: Binds to an epitope located within the peptide sequence PGDKAGFRVP as determined by overlapping synthetic peptides.
Isotype: IgG
Antibody clone number: CL0654
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

ZNF703 protein structure - AMAb90789 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human fallopian tube and liver tissues using AMAb90789 antibody. Corresponding ZNF703 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human breast (ductal carcinoma) shows strong nuclear immunoreactivity in cancer cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human breast (lobular carcinoma) shows strong nuclear immunoreactivity in cancer cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human colon (colorectal adenocarcinoma) shows moderate nuclear positivity in cancer cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human stomach (ventricle cancer) shows strong nuclear positivity in cancer cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human small intestine shows strong nuclear immunoreactivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human placenta shows strong nuclear immunoreactivity in the trophoblasts.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human liver shows absence of immunoreactivity in hepatocytes (negative control).

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human breast cancer shows moderate to strong nuclear positivity in tumor cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human colorectal cancer shows strong nuclear positivity in tumor cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human fallopian tube shows strong nuclear positivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human small intestine shows moderate to strong nuclear positivity in glandular cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
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Experimental details: Immunohistochemical staining of human liver shows no nuclear positivity in hepatocytes as expected.