PA5-28640
antibody from Invitrogen Antibodies
Targeting: ZNF703
FLJ14299, NLZ1, ZEPPO1, ZNF503L, Zpo1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-28640 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZNF703 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, A431, ZNF703-transfected 293T.
- Concentration
- 0.58 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZNF703 using 30 µg of 293T lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZNF703 polyclonal antibody (Product # PA5-28640) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ZNF703 using 30 µg of A) Non-transfected HeLa lysates and B) ZNF703 transfected HeLa lysates. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZNF703 polyclonal antibody (Product # PA5-28640) at a dilution of 1:5000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZNF703 was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZNF703 Polyclonal Antibody (Product # PA5-28640) at a dilution of 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ZNF703 was performed by separating 30 µg of various whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZNF703 Polyclonal Antibody (Product # PA5-28640) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZNF703 Polyclonal Antibody detects ZNF703 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZNF703 protein stained by ZNF703 Polyclonal Antibody (Product # PA5-28640) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ZNF703 Polyclonal Antibody detects ZNF703 protein at cytosol and nucleus on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. ZNF703 Polyclonal Antibody (Product # PA5-28640) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.