Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-32305 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal Prohibitin Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 400 ul
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Prohibitin Antibody (1215CT487.109.106) [NBP2-32305] - Lysates from 293, MCF-7, HepG2, mouse NIH/3T3 cell line and rat liver tissue (from left to right) using USF1 Antibody (Center). NBP2-32305 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: Prohibitin Antibody (1215CT487.109.106) [NBP2-32305] - Analysis of paraffin-embedded Human stomach diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.